Composite
Part:BBa_K2201343:Design
Designed by: Svenja Vinke Group: iGEM17_Bielefeld-CeBiTec (2017-10-20)
Fusion protein of CFP and YFP with an amber codon in the linker under T7-promotor control
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 770
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1360
Illegal AgeI site found at 1472 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fusion protein was generated via gibson assembly.
Source
BBa_K592101 and BBa_K404319